Nucleosome, Recombinant Human, H2AXS139phos dNuc, Biotinylated

Catalogue Number: 16-0366-EPC

Manufacturer:EpiCypher
Shelf Life:6 months
Type:Nucleosome
Host Cell:E. coli & synthetic DNA|Human
Shipping Condition:Dry Ice
Unit(s): 50 ug
Host name: Human
Clone:
Isotype:
Immunogen:

Description

Description: Recombinant mononucleosomes (H2AXS139phos) consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2AX, H2B, H3.1 and H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. H2AXS139phos dNuc contains phosphorylated-serine at position 139 on histone H2AX. The DNA in this nucleosome contains a 5' biotin-TEG group.

Additional Text

Modification

Phosphorylated, Ser139

Application Notes

H2AXS139phos mononucleosome is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments.

Storage Note

Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws