Nucleosome, Recombinant Human, H3K27bu dNuc, Biotinylated

Catalogue Number: 16-0384-EPC

Manufacturer:EpiCypher
Shelf Life:6 months
Type:DNA Binding Proteins
Shipping Condition:Dry Ice
Unit(s): 50 ug

Description

Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary synthetic method) contains butyryl-lysine at position 27. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group.

Additional Text

Modification

Butyrylated, Lys27

Application Notes

Nucleosome, Recombinant Human, H3K27bu dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. Nucleosome, Recombinant Human, H3K27bu dNucs from EpiCypher does not contain free DNA which could alter assayed activities.