Catalogue Number: 16-0386-EPC
|Shelf Life:||6 months|
|Type:||DNA Binding Proteins|
|Shipping Condition:||Dry Ice|
Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H2B (created by a proprietary synthetic method) contains acetyl-lysine at position 12. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group.
Nucleosome, Recombinant Human, H2BK12ac dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments.