Nucleosome, Recombinant Human, H2AK119ub1 dNuc, Biotinylated
Catalogue Number: 16-0395-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 25 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: H2AK119ub1 designer mononucleosomes (dNucs) consist of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The dominant form of ubiquitinated histones are monoubiquitinated H2A and H2B. Ubiquitination of H2A is usually correlated with transcriptional repression, while ubiquitination of H2B is more frequently correlated with transcriptional activation. Histone ubiquitination can be induced by DNA damage, therefore playing an important role in DNA damage response. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA in this nucleosome contains a 5' biotin-TEG group.
Additional Text
Modification
Ubiquitinated, Lys119
Application Notes
H2AK119ub1 dNuc is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
16-0395 Datasheet
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