Nucleosome, Recombinant Human, H3K14ub1 dNuc, Biotinylated
Catalogue Number: 16-0398-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 25 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: H3K14ub1 designer mononucleosomes (dNucs) consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1 and H4) to form a nucleosome, the basic repeating unit of chromatin. H3K14ub1 has been shown to promote the activity of the methyltransferase Clr4, which methylates H3K9, a known repressive mark [1]. The 147 bp 601 sequence, identified by Lowary & Widom [2], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA in this nucleosome contains a 5' biotin-TEG group. H3K14ub1 has a Cys to Ala substitution at position 110.
Additional Text
Modification
Ubiquitinated, Lys14
Application Notes
H3K14ub1 dNuc is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
Preparation
25 microgram nucleosome in frozen liquid in a screw cap plastic vial
16-0398 Datasheet
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