H2AK129ub1 Recombinant Nucleosome, Biotinylated
Catalogue Number: 16-0400-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 25 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Histone ubiquitination is a post-translational modification (PTM) wherein ubiquitin is added to a lysine residue of a histone protein. In combination with other PTMs, histone ubiquitination constitutes the "histone code," acting as a language read by proteins to regulate chromatin structure and gene expression. Ubiquitin is added through the sequential actions of E1 activating, E2 conjugating, and E3 ligase enzymes and is removed by deubiquitinating enzymes (DUBs). Histone ubiquitination plays an integral role in DNA damage response and has been implicated in transcriptional regulation and DNA replication [1]. Recombinant nucleosomes containing ubiquitinated histones are useful to study the biological functions of histone ubiquitination. H2AK129ub1 (histone H2A lysine 129 ubiquitination) Recombinant Nucleosome, Biotinylated consists of 147 base pairs of 601 sequence DNA [2] wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The DNA contains a 5' biotin-TEG group. H2AK129ub1 contains ubiquitinated lysine at position 129 on histone H2A. H2AK129 is ubiquitinated by E3 ligase BRCA1/BARD1, and H2AK129ub1 recruits chromatin remodeler SMARCAD1, which promotes DNA end resection as part of the homologous recombination (HR) DNA repair pathway [1].
Additional Text
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
Application Notes
H2AK129ub1 mononucleosome is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments. For a corresponding unmodified control, we recommend EpiCypher 16-0006.
Preparation
25 microgram nucleosome in frozen liquid in a screw cap plastic vial
16-0400 Datasheet
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