Nucleosome, Recombinant Human, H3K4,K9me3 dNuc, Biotinylated
Catalogue Number: 16-0402-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Recombinant mononucleosomes (H3K4,K9me3) consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of histones H2A, H2B, H3.2 and H4). The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. H3K4,K9me3 dNuc contains trimethylated-lysines at positions 4 and 9 on histone H3.2. H3K4,K9me3 has a Cys to Ala substitution at position 110. The DNA contains a 5' biotin-TEG group.
Additional Text
Modification
Trimethylated, Lys9
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
Application Notes
H3K4,K9me3 dNuc is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments.
Preparation
50 microgram nucleosome in frozen liquid in a screw cap plastic vial
16-0402 Datasheet
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