Heterotypic [H3K4me3 • H3K14ac] Recombinant Nucleosome, Biotinylated
Catalogue Number: 16-0411-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 25 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Heterotypic nucleosomes, also referred to as "asymmetric nucleosomes," contain sister histones with distinct histone variants and/or post-translational modifications (PTMs). In homotypic nucleosomes, or "symmetric nucleosomes," each pair of sister histones bears the same PTM, set of PTMs, or histone variant. Histone-modifying enzymes, chromatin remodelers, and histone chaperones differentially modify sister histones or exchange unique histone variants to form heterotypic nucleosomes. Heterotypic nucleosomes have been found at promoters of developmental genes in undifferentiated embryonic stem cells and transcription start sites (TSSs) of approximately half of the genes in budding yeast [1]. Heterotypic nucleosomes represent an additional layer of the histone code, acting as substrates for multivalent reader proteins, participating in PTM crosstalk mechanisms, and influencing reader protein binding affinity through varying local target concentration. Recombinant heterotypic nucleosomes are useful for studying chromatin dynamics and transcriptional regulation. Heterotypic [H3K4me3 • H3K14ac] Recombinant Nucleosome, Biotinylated is a fully defined semi-synthetic nucleosome containing combinatorial PTMs in trans whereby one histone H3 is trimethylated at lysine four and the other is acetylated at lysine 14. [H3K4me3 • H3K14ac] nucleosome consists of 147 base pairs of 601 sequence DNA [2] wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3.2, and H4) to form a nucleosome, the basic repeating unit of chromatin. The DNA contains a 5' biotin-TEG group, and histone H3.2 has a Cys to Ala substitution at position 110. The NURF chromatin remodeling complex subunit BPTF engages heterotypic target PTMs in cis [H3 • H3K4me3,K14ac] as opposed to trans [H3K4me3 • H3K14ac] through tandem PHD finger and bromodomain (PHD-BD) reader interactions [3].
Additional Text
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
Application Notes
Heterotypic [H3K4me3 • H3K14ac] nucleosome is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments. Trace amounts of TEV protease may be present (less than 5% of the preparation by densitometry, Figure 1). For a corresponding homotypic control, we recommend EpiCypher 16-0316 and/or EpiCypher 16-0343.
Preparation
25 microgram nucleosome in frozen liquid in a screw cap plastic vial
16-0411 Datasheet
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