Tailless Recombinant Nucleosome with Linker DNA, Biotinylated
Catalogue Number: 16-2027-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Recombinant mononucleosomes consist of 199 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 5' biotin-TEG DNA consists of a core 147 bp 601 nucleosome assembly sequence [1] flanked by 26 bp linker sequences as underlined below. After assembly, histone tails were enzymatically removed. This product is ideal for use as a negative control in binding assays and pull-down experiments.
Additional Text
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
Application Notes
Tailless Recombinant Nucleosome with Linker DNA is highly purified and suitable for use as a negative control or substrate in enzyme screening assays and nucleosome binding experiments. The biotinylated DNA enables affinity binding applications.
Preparation
50 microgram nucleosome in frozen liquid in a screw cap plastic vial
16-2027 Datasheet
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