Mononucleosomes, Recombinant, Hemi-methylated 199x601 DNA, Biotinylated
Catalogue Number: 16-2043-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
Description
Description: Mononucleosomes assembled from recombinant histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P06897; H2B-P02281; H3-Q92133; H4-P62799) wrapped by 199 base pairs of DNA containing the 601 positioning sequence DNA. The the 199bp DNA sequence contains a centrally positioned 601 sequence, identified by Lowary and Widom [1], which is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly. The 601 sequence is flanked by a hemi-methylated 26 bp sequence as shown in application notes and contains a 5' biotin-TEG group.
Additional Text
Modification
Hemi-methylated
Application Notes
DNA sequence 5'-Biotin-TEG GGACCCTATACGCGGCCGCCGAATTCCTGGAGAATCCCGGTCTGCAGGCCGCTCAATTGGTCGTAGACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATATATACATCCTGTGGATCCGCCGGTCGCGAACAGCGACC3' 3'CCTGGGATATGCGCCGGCGGCTTAAGGACCTCTTAGGGCCAGACGTCCGGCGAGTTAACCAGCATCTGTCGAGATCGTGGCGAATTTGCGTGCATGCGCGACAGGGGGCGCAAAATTGGCGGTTCCCCTAATGAGGGATCAGAGGTCCGTGCACAGTCTATATATGTAGGACACCTAGGCGGCCAGCGCTTGTCGCTGG5'
Storage Note
Stable for six months at -20°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
Preparation
50 microgram nucleosome in frozen liquid in a screw cap plastic vial
16-2043 Datasheet
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