Mononucleosomes, Recombinant, Symmetrically Methylated 199x601 DNA, Biotinylated
Catalogue Number: 16-2045-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Recombinant mononucleosomes consist of 199 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 601 sequence, identified by Lowary and Widom [1], is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly. The 601 sequence is flanked by a symmetrically methylated (bolded/blue) 26 bp sequence (underlined) as shown in the DNA sequence below. The DNA in this nucleosome contains a 5' biotin-TEG group.
Additional Text
Application Notes
Mononucleosomes, Recombinant, 199x601 DNA, Biotinylated are suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments.
Storage Note
Stable for six months at -20°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
Preparation
50 microgram nucleosome in frozen liquid in a screw cap plastic vial
16-2045 Datasheet
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