Dinucleosomes, Recombinant Human, Non-Biotinylated
Catalogue Number: 16-3104-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 25 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Products in EpiCypher's IDEA Toolbox (Innovation and Discovery of Epigenetic Applications) offer access to reagents without known or fully defined uses, enabling researchers to explore cutting-edge applications. Due to their novelty and unexplored potential, EpiCypher will engage in limited technical support. Dinucleosomes are comprised of two recombinant octamers (each containing two copies of histone proteins H2A, H2B, H3.1, and H4) positioned on a single, 442 base pair DNA template. Mononucleosomes are the basic repeating unit of chromatin; however, a dinucleosome substrate enables studying longer range chromatin interactions. The DNA contains two 601 positioning sequences (underlined, below) separated by a 60 base pair linker. The 601 sequence, identified by Lowary & Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly.
Additional Text
Protein Tag
Untagged
Application Notes
Dinucleosomes are highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
Preparation
25 microgram nucleosome in frozen liquid in a screw cap plastic vial
16-3104 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations