EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, 50-N-66, Biotinylated
Catalogue Number: 16-4114-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, 50-N-66, Biotinylated consists of 263 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 263 base pair DNA sequence includes an upstream 50 bp linker, the 147 bp ST601 nucleosome positioning sequence, identified by Lowary and Widom [1], and a downstream 66 bp linker (hereby named 50-N-66 nucleosome). One copy of the 'GATC' DNA motif is embedded within the ST601 sequence and there is a 5' biotin-TEG group. The nucleosome is a suitable target for recognition and substrate for remodeling reactions by various chromatin interacting proteins.
Additional Text
Application Notes
This product is a template for nucleosome remodeling assays using the restriction enzyme DpnII to determine accessibility of GATCs which is masked in its native configuration (prior to remodeling).
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
16-4114 Datasheet
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