EpiDyne®-FRET Nucleosome Remodeling Assay Substrate
Catalogue Number: 16-4201-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
Description
Description: This product provides a high throughput fluorescent readout of nucleosome remodeling when paired with enzymatically active remodeling complexes, such as SMARCA4, SMARCA2, and ACF (EpiCypher 15-1014, 15-1015, 15-1013). The EpiDyne®-FRET mononucleosome is assembled from recombinant human histones expressed in E. coli (two each of histones H2A-Cy5, H2B, H3.2 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3; H4-P62805) wrapped with a 212 base-pair DNA template bearing a 5' Cy3, the Widom 601 nucleosome positioning element [1], and a DpnII restriction enzyme motif (GATC) that is exposed upon remodeling. H2A has a Thr to Cys substitution at residue 120, where Cy5 is conjugated. H3.2 has a Cys to Ala substitution at residue 110.
Additional Text
Application Notes
This product is a template for nucleosome remodeling assays using Cy3/Cy5 FRET or using the restriction enzyme DpnII to determine accessibility of GATC, which is masked in its native configuration (prior to remodeling).
Storage Note
Stable for six (6) months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
16-4201 Datasheet
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