dCypher K-MetStat & OncoStat Nucleosome Panel

Catalogue Number: 16-9002-EPC

Shelf Life:6 months
Type:DNA Binding Proteins
Shipping Condition:Blue Ice
Unit(s): 96 well plate


Description: A 96-well plate containing 24 unique mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908, H2B-O60814, H3.1-P68431/H3.2-Q71DI3/H3.3-P84243, H4-P62805). Single and combinatorial histone post-translational modifications (PTMs) are created using a proprietary synthetic method. The nucleosomes are wrapped by 147 base pairs DNA containing a central 601-positioning sequence (Lowary and Widom 1998 J Mol Biol. (1):19-42) with a 5' biotin-TEG group. All nucleosomes in the panel are subjected to EpiCypher's rigorous quality control metrics, including: ESI-TOF mass spectrometry analysis of the modified proteins; SDS-PAGE to confirm octamer composition and purity; native PAGE to confirm nucleosome assembly and lack of free DNA; and Western blot analysis of the PTM or histone mutation (if applicable). For the full list of nucleosomes in the panel, including individual catalog numbers of full-size (50 µg) products, see the associated excel sheet on the product page at www.epicypher.com.

Additional Text

Application Notes

The access to epigenetic diversity in a physiological nucleosome context enables broad end-user applications, including nucleosome binding studies (e.g. chromatin reader binding preferences, see application data below for example using HP1≤ CHD), enzyme screening assays (e.g. identification of preferred substrates), and antibody specificity testing (e.g. for applications that require antibody specificity in a nucleosome, rather than histone peptide context). The biotin group on the DNA facilitates pull-down experiments. EpiCypher mononucleosomes do not contain free DNA which could alter assayed activities.


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