Description

Description: ectins are a small family of Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that control cell adhesion, proliferation, and migration (1, 2, 3). The name Nectin derives from the Latin word necto, which means "to connect". The Nectin family contains four members (Nectin-1 to -4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1y which is secreted), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such have been given the alternate name of poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus (1). Mouse Nectin-2 is a 70 to 78kD type I TM glycoprotein that is found on a variety of cell types (4, 5). It has two splice forms (4, 6, . 7). Nectin-2a/PRR2 is a 65kD short form and is synthesized as a 467 amino acid precursor. It contains a 31aa signal sequence, a 315aa extracellular domain (ECD), a 28aa TM segment, and a 93aa cytoplasmic region. The ECD contains one N-terminal V-type Ig domain and two 85-95aa C2-type Ig-like domains (aa153-337) (8). The V-domain is believed to mediate Nectin binding to its ligands (9). A long, 78kD, 530aa isoform of mouse Nectin-2 (Nectin-2) also exists. It has the same signal sequence and extracellular domain as Nectin-2a (aa1-338), but differs in the TM segment (21aa in length) and cytoplasmic region (159aa in length) (4, 6, 7). Mouse Nectin-2 ECD (aa32-338) shares 72%, 77% and 95% aa identity with the ECD in human, canine and rat Nectin-2, respectively. Nectin-2 is known to bind pseudorabies virus, and herpes simplex virus-2 (HSV-2). It also binds select HSV-1 strains. It does not bind poliovirus (1, 10, 11). As a cell adhesion molecule, Nectin-2 will form cis-homodimers (same cell) and trans-homodimers (across cells). Nectin-2 will not cis-dimerize with other Nectins, but will trans-heterodimerize with Nectin-3 and CD266/DNAM-1 (1, 3, 11, 12, 13). Nectin-2 is found concentrated at cell-to-cell interfaces, and is presumed to contribute to tight and adherens junction formation (14). Through its interaction with NK and T cell expressed DNAM-1, it also promotes lymphocyte cytotoxicity and cytokine secretion against both tumors and dendritic cells (DC) expressing Nectin-2 (15, 16). In the case of DC, this may be a mechanism whereby the immune system eliminates DC that are inefficient at antigen presentation. Nectin-2 is expressed on epithelium, endothelial cells, Sertoli cells, monocytes, dendritic cells, granulosa cells, mast cells, eosinophils and fibroblasts.

Additional Text

Caution

FOR RESEARCH USE ONLY

Uniprot ID

P32507

Specificity

Recognizes rat Nectin-2/CD112. Species Crossreactivity: mouse. No cross-reactivity with recombinant human Nectin-2 or recombinant mouse CD155/PVR is observed in direct ELISA.

Gene ID

19294

Gene Name

NECTIN2

Purification

Protein A purified

Antibody Clonality

Monoclonal