GABA ELISA, 96 wells, "RUO"
Catalogue Number: 17-GABHU-E01-ALP
| Manufacturer: | Alpco Diagnostics |
| Type: | Human ELISA Kit |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 96 Wells |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Range: | 15 - 1500 ng/ml |
| Sensitivity: | 7.5 ng/ml |
| Sample type: | Cell Culture, Plasma, Serum, Urine |
| Sample size: | 300 µL |
| Application: | ELISA, IA |
Description
Description: After extraction and derivatization Gamma-aminobutyric acid (GABA) is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized analyte concentrations of the standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standards.
Additional Text
Regulatory Statuses
Research Use Only. Not for Use in Diagnostic Procedures.
Short Description
The GABA ELISA is an enzyme immunoassay for the quantitative determination of Gamma-aminobutyric acid (GABA) in urine, plasma, serum, cell cultures and tissue homogenates.
Storage Note
store at 2-8C
17-GABHU-E01 Protocol
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