Glutamate ELISA, 96 wells, RUO
Catalogue Number: 17-GLUHU-E01-ALP
| Manufacturer: | Alpco Diagnostics |
| Type: | Human ELISA Kit |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 96 Wells |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Range: | 0.6 - 60 µg/ml |
| Sensitivity: | 0.3 µg/ml |
| Sample type: | Cell Culture, Plasma, Serum, Urine |
| Sample size: | 100 µL |
| Application: | ELISA, IA |
Description
Description: After extraction and derivatisation Glutamate is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated analyte concentrations in the standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standards.
Additional Text
Regulatory Statuses
Research Use Only. Not for Use in Diagnostic Procedures.
Short Description
The Glutamate ELISA ia an enzyme immunoassay (ELISA) for the quantitative determination of Glutamate in urine, plasma, serum, cell cultures and tissue homogenates.
Storage Note
store at 2-8C
17-GLUHU-E01 Protocol
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