Metanephrine ELISA
Catalogue Number: 17-MTPHU-E01-ALP
| Manufacturer: | Alpco Diagnostics |
| Type: | Human ELISA Kit |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 96 wells |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Range: | 10.5 - 2,000 ng/ml |
| Sensitivity: | 10.5 ng/ml |
| Sample type: | Urine |
| Sample size: | |
| Application: | ELISA, IA |
Description
Description: During the sample preparation metanephrine (metadrenaline) is quantitatively acylated. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.
Additional Text
Storage Note
store at 2-8C
17-MTPHU-E01 Protocol
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