Catalogue Number: 17404-AAT
| Manufacturer: | AAT Bioquest |
| Type: | Nucleic Acid Labeling Kits |
| Shipping Condition: | Dry Ice |
| Unit(s): | 10 reactions |
Description: ReadiLink™ iFluor® 647 Nick Translation dsDNA Labelling Kit provides a simple and efficient way to label a double stranded DNA sample with the bright and photostable iFluor® 647 dye. The labelling kit provides all necessary reagents for a complete workflow required for DNA labelling. This method utilizes a combination of DNAse and DNA polymerase to nick one strand of the DNA helix, to which iFluor 647 dye is conjugated. In addition, the kit allows the user to optimize incorporation and product size by adjusting the ratio of iFluor 647-dUTP conjugate to dTTP. It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA. The resulted iFluor® 647-labeled DNAs can be used in a variety of molecular biology techniques such as fluorescence in situ hybridization (FISH).
Multiple;|12 months