TWO-PRO™ 3 [equivalent to TO-PRO®-3] *5 mM DMSO Solution* *CAS 157199-63-8*
Catalogue Number: 17572-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Cell Stains and Indicators |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.2 ml |
Description
Description: TWO-PRO™-3 is chemically equivalent to TO-PRO®-3 (TO-PRO® is the trademark of Invitrogen). TWO-PRO™-3 is a carbocyanine dimer with far-red fluorescence similar to Cy® 5 dyes. It is cell-impermeant and easily distinguished from fluorescein and rhodamine as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. TWO-PRO™-3 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Green™ LCS1 dye and cell-impermeant TWO-PRO™-3 can be used to assess cell viability. TWO-PRO™-3 and Nuclear Green™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
17572 Protocol
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