DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7*
Catalogue Number: 17575-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Fluorescent Reagents |
| Shipping Condition: | RT |
| Unit(s): | 0.2 ml |
Description
Description: DiTO™-1 is chemically equivalent to TOTO®-1 (TOTO® is the trademark of Invitrogen). DiTO™-1 is a carbocyanine dimer with green fluorescence similar to FITC. It is cell-impermeant and easily distinguished from Cy5 and rhodamines as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiTO™-1 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Red™ LCS1 dye and cell-impermeant TOTO®-1 can be used to assess cell viability. DiTO™-1 and Nuclear Red™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
17575 Protocol
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