Nucleosome Assembly 601 Sequence DNA, 199 bp, Biotinylated
Catalogue Number: 18-2044-EPC
| Manufacturer: | EpiCypher |
| Physical state: | Lyophilized |
| Type: | Nucleosome |
| Host Cell: | Synthetic DNA |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
Description
Description: Nucleosome Assembly 601 Sequence DNA, 199 bp, Biotinylated is a double-stranded DNA fragment with 26 bp linkers on either end of a 147 bp 601 Widom sequence, identified by Lowary and Widom using the SELEX method [1]. The 601 sequence DNA has high affinity for histone octamers and is useful for in vitro nucleosome assembly. The DNA contains a 5' biotin-TEG group.
Additional Text
Application Notes
Nucleosome Assembly 601 Sequence DNA, 199 bp, Biotinylated is useful for assembly of nucleosomes using purified or recombinant histone octamers (EpiCypher 16-0001). See Luger et al. [2] for recommended nucleosome reconstitution protocol. This product also serves as a DNA only control for experiments utilizing the 199x601 nucleosome (EpiCypher 16-2044). These products are part of the Methyl DNA designer nucleosome set (epicypher.com/products/nucleosomes/methyl-dna-designer-nucleosomes).
Storage Note
Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C
Preparation
lyophilized|50 microgram lyophilized DNA in a screw cap plastic vial
18-2044 Datasheet
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