EpiDyne® Remodeling Assay Substrate DNA ST601-GATC0, Biotinylated
Catalogue Number: 18-4110-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 24 months |
| Physical state: | Lyophilized |
| Type: | Substrate |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
Description
Description: EpiDyne® Remodeling Assay Substrate DNA ST601-GATC0 is a 217 base-pair double-stranded DNA fragment containing the 601 nucleosome positioning sequence [1], which has high affinity for histone octamers and is useful for nucleosome assembly. The DNA also includes a 3' acceptor sequence to accommodate the histone octamer subsequent to remodeling and a 5' biotin-TEG group. This negative control does not contain DpnII restriction sites. When paired with the positive control DNA (EpiCypher 18-4111) these controls illustrate the migration range for the Restriction Enzyme Assay. See the EpiDyne Nucleosome Remodeling Assay Tech Note (Restriction Enzyme Assay) for more information: epicypher.com/resources/technical-notes
Additional Text
Application Notes
ST601-GATC0 DNA is useful as a negative control for restriction enzyme accessibility nucleosome remodeling assays using the EpiDyne Remodeling Assay Substrate. No DpnII restriction enzyme site is present, while the naturally occurring MfeI (AATTGG in bold) remains present within the 601 sequence.
Storage Note
Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C.
Preparation
lyophilized|50 microgram lyophilized DNA in a screw cap plastic vial
18-4110 Datasheet
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