EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1, Biotinylated
Catalogue Number: 18-4111-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 24 months |
| Physical state: | Lyophilized |
| Type: | Substrate |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
Description
Description: EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1 is a 217 base-pair double-stranded DNA fragment containing the 601 nucleosome positioning sequence [1], which has high affinity for histone octamers and is useful for nucleosome assembly. The DNA also includes a 3' acceptor sequence to accommodate the histone octamer subsequent to remodeling and a 5' biotin-TEG group. This positive control has a DpnII restriction site within the 601 sequence. When paired with the negative control DNA (EpiCypher 18-4110) these controls illustrate the migration range for the Restriction Enzyme Assay. See the EpiDyne Nucleosome Remodeling Assay Tech Note (Restriction Enzyme Assay) for more information: epicypher.com/resources/technical-notes
Additional Text
Application Notes
ST601-GATC1 DNA is useful as a positive control for restriction enzyme accessibility nucleosome remodeling assays using the Biotinylated EpiDyne Remodeling Assay Substrate, as a DpnII (GATC in red) restriction enzyme site are present within the 601 sequence. The naturally occurring MfeI restriction site(AATTGG in bold) remains present as well.
Storage Note
Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C.
Preparation
lyophilized|50 microgram lyophilized DNA in a screw cap plastic vial
18-4111 Datasheet
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