EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated
Catalogue Number: 18-4112-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 24 months |
| Physical state: | Lyophilized |
| Type: | Substrate |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
Description
Description: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated is a 217 base-pair double-stranded DNA fragment containing a 5' biotin-TEG group and two GATC restriction enzyme sites. This sequence includes the 601 nucleosome positioning sequence [1], which has high affinity for histone octamers and is useful for nucleosome assembly. The DNA also includes a 3' acceptor sequence to accommodate the histone octamer subsequent to remodeling and a 5' biotin-TEG group. When assembled onto a nucleosome, the GATC sites are shielded within the 601 sequence but can be made accessible for restriction enzyme cleavage after nucleosome remodeling. See the EpiDyne Nucleosome Remodeling Assay Tech Note (Restriction Enzyme Assay) for more information: epicypher.com/resources/technical-notes
Additional Text
Application Notes
ST601-GATC1,2 DNA is useful as naked DNA template control for nucleosome remodeling assays using the EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2 (EpiCypher 16-4112). See the EpiDyne Restriction Enzyme Assay Technical Note for more information: epicypher.com/resources/technical-notes/.
Storage Note
Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C.
Preparation
lyophilized|50 microgram lyophilized DNA in a screw cap plastic vial
18-4112 Datasheet
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