EpiDyne™-FRET Remodeling Assay Substrate DNA
Catalogue Number: 18-4201-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 24 months |
| Physical state: | Lyophilized |
| Type: | Substrate |
| Host Cell: | Synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: EpiDyne™-FRET Remodeling Assay Substrate DNA is a 207 base-pair double-stranded Cy3-conjugated DNA fragment. This sequence includes the Lowary 601 nucleosome positioning sequence [1] (see EpiCypher 18-0005), a 5' Cy3 fluorophore, as well as a 3' acceptor sequence to accommodate the histone octamer subsequent to remodeling. The DNA also has a restriction enzyme site embedded in the 601 sequence that is accessible to the DpnII restriction enzyme after nucleosome remodeling.
Additional Text
Storage Note
Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C.
Preparation
lyophilized|50 microgram lyophilized DNA in a screw cap plastic vial
Application Notes
EpiDyne™-FRET Remodeling Assay Substrate DNA is the DNA component of EpiDyne™-FRET Remodeling Assay Substrate (EpiCypher 16-4201) and can be used for nucleosome remodeling assays using Cy3/Cy5 FRET or using the restriction enzyme DpnII to determine accessibility of GATC, which is masked in its native configuration (prior to remodeling).
18-4201 Datasheet
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