SNAP-ChIP® OncoStat Full Panel Primer Set

Catalogue Number: 18-6201-EPC

Shelf Life:24 months
Shipping Condition:Blue Ice
Unit(s): 100 reactions


Description: The SNAP-ChIP OncoStat Full Panel Primer Set contains a universal forward primer pre-mixed with reverse primers that uniquely amplify DNA-barcoded nucleosomes in the SNAP-ChIP OncoStat Panel (Catalog No. 19-2001), consisting of H3.3 wild-type (WT), H3.3K4M, H3.3K9M, H3.3K27M, H3.3G34R, H3.3G34V, H3.3G34W, and H3.3K36M. These primers can be used in SNAP-ChIP experiments where quantitative PCR (qPCR) provides an assessment of antibody performance and technical variability. The primers are compatible with SYBR chemistry. For TaqMan applications, the universal SNAP-ChIP Dual Label Hydrolysis Probe can be purchased separately (Catalog No. 18-6001). The volume is sufficient to perform 100 x 10 µL qPCR reactions (see SNAP-ChIP manual).

Additional Text

Application Notes

For use in SNAP-ChIP-qPCR. Compatible with the SNAP-ChIP OncoStat Panel (Catalog No. 19-2001) and the SNAP-ChIP Dual Label Hydrolysis Probe (Catalog No. 18-6001). DNA Sequence: SNAP-ChIP OncoStat Forward Primer 5'-CGTATCGCGCGCATAATA-3' H3.3 WT Reverse Primer 5'-ATACGACGAGATAGTCGACG-3' H3.3K4M Reverse Primer 5'-TCGCGCGTACGAAAC-3' H3.3K9M Reverse Primer 5'-ATTCGCGCGTACGTATAC-3' H3.3K27M Reverse Primer 5'-ACGCGATAACGTCGACTA-3' H3.3G34R Reverse Primer 5'-TATCGCGTCGTACGATCG-3' H3.3G34V Reverse Primer 5'-CGCGATTCGCGTAATACG-3' H3.3G34W Reverse Primer 5'-GCGACGCGTAATCGA-3' H3.3K36M Reverse Primer 5'-TAATCGACGCGTTACGC-3'


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