SNAP-ChIP® K-MetStat Panel

Catalogue Number: 19-1001-EPC

Shelf Life:6 months
Type:DNA Binding Proteins
Shipping Condition:Blue Ice
Unit(s): 20 ul


Description: A panel of distinctly modified mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431 or H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pairs of barcoded Widom 601 positioning sequence DNA. The mononucleosomes constitute a pool of 1 unmodified plus 15 histone H3 or H4 post-translational modifications (PTMs, created by a proprietary semi-synthetic method): H3K4me1, H3K4me2, H3K4me3, H3K9me1, H3K9me2, H3K9me3, H3K27me1, H3K27me2, H3K27me3, H3K36me1, H3K36me2, H3K36me3, H4K20me1, H4K20me2 and H4K20me3. Each distinctly modified nucleosome is distinguishable by a unique sequence of DNA ( barcode,) at the 3' end that can be deciphered by qPCR or next-generation sequencing. Each of the 16 nucleosomes in the pool is wrapped by 2 distinct DNA species, each containing a distinct barcode (,A, and B, see SNAP-ChIP Manual) allowing for an internal technical replicate. * Histone H3.2 contains a Cys to Ala substitution at position 110.

Additional Text

Application Notes

SNAP-ChIP K-MetStats are highly purified recombinant mononucleosomes and are suitable for use as spike-in controls for ChIP reactions, for antibody specificity testing or for effector protein binding experiments. See manual for more information.