SNAP-ChIP® K-MetStat™ Panel
Catalogue Number: 19-1001-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | DNA Binding Proteins |
| Shipping Condition: | Blue Ice |
| Unit(s): | 20 ul |
Description
Description: A panel of distinctly modified mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431 or H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pairs of barcoded Widom 601 positioning sequence DNA. The mononucleosomes constitute a pool of 1 unmodified plus 15 histone H3 or H4 post-translational modifications (PTMs, created by a proprietary semi-synthetic method): H3K4, K9, K27, and H4K20 with me1, me2, or me3. Each distinctly modified nucleosome is distinguishable by a unique sequence of DNA ("barcode") at the 3' end that can be deciphered by qPCR or next-generation sequencing. Each of the 16 nucleosomes in the pool is wrapped by 2 distinct DNA species, each containing a unique barcode ("A" and "B", see SNAP-ChIP Manual) allowing for an internal technical replicate. * Histone H3.2 contains a Cys to Ala substitution at position 110.
Additional Text
Application Notes
SNAP-ChIP K-MetStat Panel consists of a pool of highly purified recombinant mononucleosomes and is suitable for use as spike-in controls for ChIP reactions, for antibody specificity testing or for effector protein binding experiments. See manual for more information.
Storage Note
Stable for six months at -20°C from date of receipt.
19-1001 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations