10x Buffer R; Strep-Tactin® Regeneration Buffer with HABA
Catalogue Number: 2-1002-100-IBA
| Manufacturer: | IBA GmbH |
| Shelf Life: | 6 Months |
| Type: | Biological Buffers |
| Shipping Condition: | RT |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ml |
Description
Description: 10x Buffer R is a 10x concentrated Strep-Tactin® regeneration buffer containing HABA. HABA (2-[4'-hydroxy-benzeneazo]benzoic acid) displaces desthiobiotin after the elution of Strep-tag®II or Twin-Strep-tag® fusion proteins from the modified biotin-binding pocket of Strep-Tactin®. The yellow buffer turns red upon binding of HABA to Strep-Tactin®, clearly indicating the regeneration process and activity status of the column. Please note that 10x Buffer R is not applicable for regeneration of Strep-Tactin®XT resins, but it can be used to monitor the activity status after the regeneration.
Additional Text
Short Description
Strep-Tactin® regeneration buffer with HABA
2-1002-100 Datasheet
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