pASK-IBA5C

Catalogue Number: 2-1324-000-IBA

Manufacturer:IBA GmbH
Shelf Life:12 Months
Type:Vector
Shipping Condition:RT
Storage Condition:2-8°C
Unit(s): 5 ug

Description

Description: pASK-IBA5C is a classic cloning vector devised for E. coli expression of recombinant proteins with the Strep-tag®II fused to the N-terminus. It carries the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI), and the inducible tetracycline promoter/operator for the regulated expression of proteins. The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. The recombinant protein will be localized in the cytoplasm.

Additional Text

Short Description

Cytoplasmic E. coli expression vector with N-terminal Strep-tag®II and chloramphenicol resistance