Epstein Barr Virus Nuclear Antigen (IgG) ELISA, (96 wells) "RUO"
Catalogue Number: 20-ENGHU-E01-ALP
| Manufacturer: | Alpco Diagnostics |
| Type: | Human ELISA Kit |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 96 Wells |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Range: | pos. / neg. control |
| Sensitivity: | |
| Sample type: | Plasma, Serum |
| Sample size: | 10 µL |
| Application: | ELISA, IA |
Description
Description: The Epstein-Barr Virus (EBNA-1) IgG ELISA Kit is a solid-phase enzyme-linked immunosorbentassay (ELISA). Microtiter wells as a solid phase are coated with recombinant EBV nuclear antigentype 1. Diluted samples and ready-for-use controls are pipetted into these wells. Duringincubation, EBNA-1-specific antibodies of positive samples and controls are bound to theimmobilized antigens. After a washing step to remove unbound sample and control material,horseradish peroxidase-conjugated anti-human IgG antibodies are dispensed into the wells. Duringa second incubation, this anti-IgG conjugate binds specifically to IgG antibodies resulting in theformation of enzyme-linked immune complexes. After a second washing step to remove unboundconjugate, the immune complexes formed (in case of presence of antigen) are detected byincubation with TMB substrate and development of a blue color. The blue color turns into yellow bystopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directlyproportional to the amount of EBNA-1-specific IggG antibody in the sample. Absorbance at 450 nmis read using an ELISA microtiter plate reader.
Additional Text
Regulatory Statuses
Research Use Only. Not for Use in Diagnostic Procedures.
Short Description
Epstein Barr Virus Nuclear Antigen IgG ELISA
Storage Note
store at 2-8C
20-ENGHU-E01 Protocol
SUPPORT
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PRODUCT
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DELIVERY
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