LH (Lutenizing Hormone) (Urine) ELISA (96 wells) "IVD"
Catalogue Number: 20-LHUHU-E01-ALP
| Manufacturer: | Alpco Diagnostics |
| Type: | Human ELISA Kit |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 96 Wells |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Range: | 0.287-200 mIU/mL |
| Sensitivity: | 0.287 mIU/mL |
| Sample type: | Urine |
| Sample size: | 25 µL |
| Application: | ELISA, IA |
Description
Description: The LH (Urine) ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) basedon the sandwich principle. The microtiter wells are coated with a monoclonal (mouse) antibodydirected towards a unique antigenic site of the β-subunit of the LH molecule. During the firstincubation, the β-subunit of the LH molecule in the added sample binds to the immobilized antibody. After incubation, the added conjugate solution, which contains an anti-LH antibodyconjugated to horseradish peroxidase, binds to the LH forming a sandwich complex. After awashing step, to remove all unbound substances, the solid phase is incubated with the substratesolution. The colorimetric reaction is abruptly stopped by addition of stop solution and the opticaldensity (OD) of the resulting yellow product is measured. The intensity of color is proportional tothe concentration of the analyte in the sample. A standard curve is constructed by plotting ODvalues against concentrations of standards, and concentrations of unknown samples aredetermined using this standard curve.
Additional Text
Regulatory Statuses
Research Use Only. Not for Use in Diagnostic Procedures.
Short Description
Luteinizing Hormone ELISA (LH ELISA) (Urine)
Storage Note
store at 2-8C
20-LHUHU-E01 Protocol
SUPPORT
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PRODUCT
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DELIVERY
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