Parvovirus B 19 (IgM) ELISA, (96 wells) "RUO"

Catalogue Number: 20-PBMHU-E01-ALP

Manufacturer:	Alpco Diagnostics
Type:	Human ELISA Kit
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	96 Wells
Host name:
Clone:
Isotype:
Immunogen:
Range:	pos. / neg. / cut off control
Sensitivity:
Sample type:	Serum
Sample size:	10 µL
Application:	ELISA, IA

Overview
Datasheets

Description

Description: The Parvovirus B19 IgM ELISA Kit is a solid-phase enzyme-linked immunosorbent assay (ELISA).Specimen samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyperimmune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoidfactors. This pretreatment avoids false results. Microtiter wells as a solid phase are coated with Parvovirus B19 antigen.Pretreated sample specimens and ready-for-use controls are pipetted into these wells. During incubation ParvovirusB19-specific antibodies of positive specimens and controls are bound to the immobilized antigens.After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgMantibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specifically to IgMantibodies resulting in the formation of enzyme-linked immune complexes.After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) aredetected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stoppingthe enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Parvovirus B19-specific IgM antibody in the samplespecimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.

Additional Text

Regulatory Statuses

Research Use Only. Not for Use in Diagnostic Procedures.

Short Description

Parvovirus B 19 IgM ELISA

Storage Note

store at 2-8C

20-PBMHU-E01 Protocol

Price

£645.00

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