Ureaplasma urealyticum (IgM) ELISA, (96 wells) "RUO"

Catalogue Number: 20-UUMHU-E01-ALP

Manufacturer:	Alpco Diagnostics
Type:	Human ELISA Kit
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	96 Wells
Host name:
Clone:
Isotype:
Immunogen:
Range:	pos. / neg. control
Sensitivity:
Sample type:	Serum
Sample size:	10 µL
Application:	ELISA, IA

Overview
Datasheets

Description

Description: The Ureaplasma urealyticum IgM ELISA kit is a solid phase enzyme-linked immunosorbentassay (ELISA). Samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent,containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition fromspecific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or falsepositive results. Microtiter wells as a solid phase are coated with Ureaplasma urealyticum UreD protein. Dilutedsamples and ready-for-use controls are pipetted into these wells. During incubation Ureaplasmaurealyticum-specific antibodies of positive samples and controls are bound to the immobilizedantigens. After a washing step to remove unbound sample and control material, horseradishperoxidase conjugated anti-human IgM antibodies are dispensed into the wells. During asecond incubation, this anti-IgM conjugate binds specifically to IgM antibodies resulting in theformation of enzyme-linked immune complexes. After a second washing step to removeunbound conjugate, the immune complexes formed (in case of positive results) are detected byincubation with TMB substrate and development of a blue color. The blue color turns yellow bystopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directlyproportional to the amount of Ureaplasma urealyticum-specific IgM antibody in the sample.Absorbance at 450 nm is read using an ELISA microtiter plate reader.

Additional Text

Regulatory Statuses

Research Use Only. Not for Use in Diagnostic Procedures.

Short Description

Ureaplasma Urealyticum IgM ELISA

Storage Note

store at 2-8C

20-UUMHU-E01 Protocol

Price

£336.00

-

Quantity
Unit Size

Find out about delivery