Fluo-4 AM *Ultrapure Grade* *CAS 273221-67-3*
Catalogue Number: 20551-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Physical state: | Liquid |
| Type: | Calcium Indicator |
| Shipping Condition: | RT |
| Unit(s): | 10x50 ug |
Description
Description: Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4 is an analog of Fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis, and require harsh cell loading conditions to maximize their cellular calcium responses. Fluo-8® and Cal-520® calcium dyes have been developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation @ ~490 nm and maximum emission @ ~520 nm.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
20551 Protocol
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