Calbryte™-520L, potassium salt
Catalogue Number: 20642-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Calcium Indicator |
| Shipping Condition: | RT |
| Unit(s): | 10 x 50 ug |
Description
Description: The intracellular calcium flux assay is a widely used method for monitoring signal transduction pathways and high throughput screening of G protein-coupled receptors (GPCRs) and calcium channel targets. Following the introduction of Fluo-3 in 1989, Fluo-4, Fluo-8, and Cal-520 were later developed with enhanced signal-to-background ratios and soon became the Ca2+ indicators of choice for confocal microscopy, flow cytometry, and high throughput screening applications. Calbryte 520L has a low affinity to calcium ions with a Kd ~91 µM. Calbryte 520L produces a bright fluorescence signal in the presence of calcium at a high concentration. It has an identical excitation and emission wavelength as Fluo-4; thus, the same Fluo-4 assay settings can be readily applied to Calbryte 520L-based calcium assays.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
20642 MSDS
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