Catalogue Number: 209-062-241-JIR
| Manufacturer: | Jackson ImmunoResearch |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Freeze-dried solid |
| Type: | Monoclonal Secondary Antibody - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 0.25 ml |
| Host name: | Mouse |
| Clone: | ME.114 |
| Isotype: | IgG2b, kappa |
| Immunogen: | IgE |
| Application: | ELISA, FC, ICC, IF, WB, IHC |
Description: Based on immunoelectrophoresis and/or ELISA, the antibody reacts with human IgE. No antibody was detected against human IgG, IgM, or IgA. The antibody may cross-react with immunoglobulins from other species.
Monoclonal
1:20,000 - 1:400,000 for ELISA and Western blotting using enzyme-conjugated streptavidin 1:500 - 1:5,000 for enzyme immunohisto/cytochemistry 1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry Not recommended for detection of reduced IgE Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.