5x ACF Remodeling Assay Buffer

Catalogue Number: 21-0013-EPC

Manufacturer:EpiCypher
Shelf Life:6 months
Type:Biological Buffers
Shipping Condition:Dry Ice
Unit(s): 500 ul

Description

Description: 5x concentrated reaction buffer for use with ACF Remodeling Enzyme Complex (Catalog No. 15-1013). Optimized for use with recombinant Drosophila ACF Remodeling Enzyme Complex, assembled from Acf1-FLAG and ISWI produced in SF9 cells. ACF is an ATP-dependent chromatin remodeling complex that regulates nucleosome spacing.

Additional Text

Protein Tag

6xHIS-FLAG™ Tag

Application Notes

This produt is formulated to perform remodeling reactions using ACF Remodeling Enzyme Complex and EpiDyne-FRET substrate in a reaction defined as 10 µl containing [5 nM ACF Complex/ 20 nM EpiDyne-FRET/ 50 µM rATP] and remodels to completion in <20 minutes. For best results, store 5x ACF Remodeling Assay buffer at 4°C and avoid freezing. For further information, please contact techsupport@epicypher.com or refer to the EpiDyne-FRET Technote (TN#106; available at www.epicypher.com/technotes).