Catalogue Number: 21-0013-EPC
|Shelf Life:||6 months|
|Shipping Condition:||Dry Ice|
Description: 5x concentrated reaction buffer for use with ACF Remodeling Enzyme Complex (Catalog No. 15-1013). Optimized for use with recombinant Drosophila ACF Remodeling Enzyme Complex, assembled from Acf1-FLAG and ISWI produced in SF9 cells. ACF is an ATP-dependent chromatin remodeling complex that regulates nucleosome spacing.
This produt is formulated to perform remodeling reactions using ACF Remodeling Enzyme Complex and EpiDyne-FRET substrate in a reaction defined as 10 µl containing [5 nM ACF Complex/ 20 nM EpiDyne-FRET/ 50 µM rATP] and remodels to completion in <20 minutes. For best results, store 5x ACF Remodeling Assay buffer at 4°C and avoid freezing. For further information, please contact firstname.lastname@example.org or refer to the EpiDyne-FRET Technote (TN#106; available at www.epicypher.com/technotes).