CUTANA™ 5% Digitonin
Catalogue Number: 21-1004-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 24 months |
| Type: | Biological Buffers |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 ml |
| Application: | CUT&RUN, CUT&Tag |
Description
Description: Digitonin is a nonionic detergent used in CUTANA™ CUT&RUN and CUT&Tag genomic mapping assays to permeabilize cells, facilitating antibody and enzyme access to chromatin. Digitonin also helps prevent clumping of Concanavalin A (ConA) magnetic beads and film formation on tubes, which minimizes sample loss. For this reason, Digitonin is recommended in assay buffers for both cell and nuclei samples. Refer to the appropriate kit manual or do-it-yourself (DIY) protocol for guidance on which assay buffers require Digitonin.
Additional Text
Storage Note
Stable for 2 years at -20°C from date of receipt.
Application Notes
CUTANA™ 5% Digitonin can be used to prepare buffers for use in CUT&RUN or CUT&Tag. If using intact cells, the concentration of Digitonin should be optimized for efficient cell permeabilization. To avoid Digitonin precipitation and cell lysis, use the minimal concentration of Digitonin required for efficient cell permeabilization. EpiCypher has found 0.01% Digitonin to be the optimal concentration for most cell types. See www.epicypher.com/protocols for detailed protocols. Visit support.epicypher.com for guidance on optimizing digitonin concentration.
Preparation
1 mililiter liquid in a screw cap plastic vial
21-1004 Datasheet
SUPPORT
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PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations