Indo-1, pentapotassium salt *CAS 132319-56-3*
Catalogue Number: 21040-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Cell Stains and Indicators |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
Description
Description: Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fura-2 and Indo-1 are most commonly used among the UV-excitable calcium indicators. Fura-2 is excitation-ratioable while Indo-1 is emission-ratioable. Fura-2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Upon binding Ca2+, Fura-2 exhibits an absorption shift that can be observed by scanning the excitation spectrum between 300 and 400 nm, while monitoring the emission at ~510 nm. In contrast, Indo-1 is the preferred dye for flow cytometry, where it is more practical to use a single laser for excitation (usually the 351–364 nm spectral lines of the argon-ion laser). The emission of Indo-1 shifts to 400 nm (when bound to Ca2+) from 480 nm in Ca2+-free environments.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
21040 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations