Anti-Vimentin Antibody
Catalogue Number: 2105-VIM-PPS
2105-VIM Image
2105-VIM Image
2105-VIM Image
2105-VIM Image
2105-VIM Image
Immunostaining of HeLa cells labelled with anti-vimentin antibody (cat.2105-VIM, 1:10,000, green), anti-actin (red), and DAPI (blue) labelling of nuclear DNA. The vimentin antibody labels the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
2105-VIM Image
Immunostaining of tumors and precursor PanIN lesions from 4 week old LSL-KrasG12D; Cdkn2alox/lox; p48Cre (KIC) mice that were treated with 2G8 and/or gemcitabine, Gem. (Anti-Vimentin, cat. 2105-VIM, green; Anti-E-cadherin, red). Photo courtesy of Rolf Brekken, University of Texas Southwestern. CC-BY-4.0
2105-VIM Image
Immunostaining of mixed neuron/glial cultures stained with anti-Vimentin antibody (cat. 2105-VIM, 1:500, green) and anti-GFAP antibody (cat.620-GFAP,1:1000, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin).
2105-VIM Image
Western blot of NIH 3T3 cell lysate showing specific immunolabeling of the ~50 kDa Vimentin protein.
| Manufacturer: | Phosphosolutions |
| Preservative: | 10 mM Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CTRCT30 antibody, Epididymis luminal protein 113 antibody, FLJ36605 antibody, HEL113 antibody, VIM antibody, VIME_HUMAN antibody, vimentin antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgY |
| Immunogen: | Recombinant human vimentin purified from E. coli. |
| Application: | ICC, WB, IHC |
Additional Text
Gene Name
VIM
Gene ID
7431
Molecular Weight
50
Uniprot ID
P08670
Antibody Clonality
Polyclonal
Short Description
Our Anti-Vimentin primary antibody from PhosphoSolutions is chicken polyclonal. It detects human, mouse, and rat Vimentin and is total IgY fraction. It is great for use in WB, IHC, ICC.
Storage Note
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
2105-VIM Datasheet
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