Rhod-5N, AM

Catalogue Number: 21070-AAT

Manufacturer:AAT Bioquest
Shelf Life:12 months
Type:Fluorescent Reagents
Shipping Condition:RT
Unit(s): 1 mg

Description

Description: Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-5N has a lower binding affinity for Ca2+ (Kd = ~320 µM) than any other BAPTA-based indicator and is suitable for Ca2+ measurements from 10 µM to 1 mM. Like the parent Rhod-2 indicator, Rhod-5N is essentially nonfluorescent in the absence of divalent cations and exhibits strong fluorescence enhancement with no spectral shift upon binding Ca2+. Rhod-5N AM is cell-permeable version of Rhod-5N.

Additional Text

Storage Note

Freeze (< -15 °C); Minimize light exposure;|12 months