Cell Meter™ Fluorimetric Intracellular pH Assay Kit
Catalogue Number: 21180-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 6 months |
| Type: | Detection Kit |
| Shipping Condition: | RT |
| Unit(s): | 1000 Tests |
Description
Description: Intracellular pH change are implicated in diverse physiological and pathological processes, including cell proliferation, apoptosis, fertilization, malignancy, multidrug resistance, ion transport, lysosomal storage disorders and Alzheimer’s disease. The Cell Meter™ Fluorimetric Intracellular pH Assay Kit utilizes AAT Bioquest’s proprietary fluorescent indicator for measuring the relative intracellular pH changes. It is a homogeneous, kinetic, live-cell fluorescent assay that utilizes either a standard procedure or acid-load procedure. The standard protocol is designed for measuring the therapeutic targets of interest with a decrease in intracellular pH upon treatment. The ‘Acid-Load’ procedure is designed to measure the increase of intracellular pH associated with changes in cellular metabolism due to GPCR activation or growth factor activity. With the ‘Acid-Load’ procedure ammonium chloride solution is added after the fluorescent pH dye is loaded into cells in a minimum volume. This ‘acid-loading’ step is followed by the addition of agonist in a relatively large volume (~4X) of buffer. The sudden volume change initiates an efflux of ammonia (NH3) from the cells causing a rapid decrease in intracellular pH, and thus a decrease in fluorescence signal. The effect of agonist on the subsequent recovery of intracellular pH is measured by the relative fluorescence signal increase.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|6 months
21180 Protocol
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