Spexyte™ Intracellular pH Calibration Buffer Kit

Catalogue Number: 21235-AAT

Manufacturer:	AAT Bioquest
Shelf Life:	24 months
Type:	Cell based assay
Shipping Condition:	RT
Storage Condition:	2-8°C
Unit(s):	100 tests

Overview
Datasheets

Description

Description: Intracellular pH (pHi) plays an important modulating role in many cellular events, including cell volume regulation, cellular metabolism, calcium regulation, receptor-mediated signal transduction, ion transport, endocytosis, and other cellular processes. Intracellular pH is generally 6.8-7.4 in the cytosol and 4.5-6.0 in the acidic organelles. Intracellular pH changes have significant physiological effects, e.g., the pH-dependent concentration of intracellular messengers such as Ca2+ and cAMP affects cellular signaling. Several recent reports showed the dysregulated pH is emerging as a hallmark of cancer cells. Spexyte™ Intracellular pH Calibration Buffer Kit provides a range of pH calibration buffers (pH 4.5-8.0) with nigericin, which modulate the intracellular pH with the external pH in the presence of 100-150 mM K+. When used in conjunction with pH indicators, such as BCFL, AM or BCECF, AM, Spexyte™ Intracellular pH Calibration Buffer Kit can create a standard curve which is used to determine the intracellular pH.

Additional Text

Storage Note

Refrigerated (2-8 °C);|24 months

21235 Protocol

21235 MSDS

Price

£223.00

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