Cytochrome B245 (p22phox, Flavocytochome B, Cyt B) (MaxLight 490)
Catalogue Number: 214778-ML490-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490. |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | CS9 |
| Isotype: | IgG1 |
| Immunogen: | Cytochrome B245 solubilized in octyl-beta-gluycopyranoside. |
| Application: | IP, WB, IHC, FLISA |
Description
Description: MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. The light chain (p22phox), along with the heavy chain (gp91phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons. The core region of the epitope is aa165-169 (KKPSE), a cytosolic epitope on p22phox not accessible on intact neutrophils. Mouse anti Human Cytochrome B245 Light Chain, also has an inhibitory effect on NADPH oxidase activation.
Additional Text
Gene Name
CYBA
Uniprot ID
P13498
Specificity
Recognizes human Cytochrome B245.
Purification
Protein G purified
Gene ID
1535
Antibody Clonality
Monoclonal
214778-ML490 Datasheet
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