N-Cadherin (CDHN, NCAD, Neural Cadherin, CD325) (MaxLight 550)

Catalogue Number: 215041-ML550-USB

Manufacturer:	United States Biological
Physical state:	Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Type:	Monoclonal Primary Antibody - Conjugated
Alias:	cadherin 2, type 1, N-cadherin (neuronal)\|cadherin-2\|calcium-dependent adhesion protein, neuronal\|CD325\|CDHN\|CDw325\|NCAD\|N-cadherin 1\|neural cadherin
Shipping Condition:	Blue Ice
Unit(s):	100 ul
Host name:	Mouse
Clone:	13A9
Isotype:	IgG1
Immunogen:	Recombinant MBP fusion protein containing the entire cytoplasmic domain of human N-cadherin.
Application:	IF, IP, WB, IHC

Overview
Datasheets

Description

Description: MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Neural cadherin (N-Cadherin), otherwise known as CD325 is a calcium dependent cell-cell adhesion glycoprotein, and member of the cadherin superfamily, which links to the actin cytoskeleton via catenins, and plays a role in cell-matrix adhesion, cell growth and differentiation, and the establishment of left-right asymmetry. N-cadherin is expressed by neurons, endothelial cells, muscle cells, and stem cells, and is one of the primary cadherins recruited to the site of neuronal synapse formation. N-cadherin is directly involved in the differentiation of early hematopoietic progenitors, and is commonly expressed by cancer cells, playing a role in transendothelial migration and metastasis, through the up-regulation of the src kinase pathway, and subsequent failure of the intercellular connection between two adjacent endothelial cells. The clone studies have demonstrated that expression levels of E-Cadherin and N-Cadherin have a role to play in the invasive properties of breast cancer. Decreased levels of E-cadherin and loss of E-cadherin-mediated adhesion, can result in the transition of a benign epithelial tumor to an invasive tumor, and increase invasiveness, whilst the expression of N-cadherin correlates with motility, invasiveness and tumor metastasis, irrespective of the presence of E-cadherin (Nieman et al. 1999). The clone has been shown to be specific for N-cadherin, and does not recognize E-cadherin, M-cadherin or P-cadherin (Knudsen et al. 1995). Immunohistological studies have shown that clone can be used as a reliable marker for the differential diagnosis of pleural mesotheliomas and lung adenocarci. The product detects a band of ~35kD-140kD in human HT-1080 and HeLa cell lysates.

Additional Text

Gene Name

CDH2

Gene ID

1000

Uniprot ID

P19022

Specificity

Recognizes human N-Cadherin. Species Crossreactivity: Rat

Purification

Protein G purified

Accession Number

NM_001792

Antibody Clonality

Monoclonal

215041-ML550 Datasheet

Price

£954.00

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