CytoCalcein™ Violet 450 *Excited at 405 nm*

Catalogue Number: 22012-AAT

Manufacturer:AAT Bioquest
Shelf Life:12 months
Type:Cell Stains and Indicators
Shipping Condition:RT
Unit(s): 1 mg

Description

Description: CytoCalcein™ Violet 450 is designed for labeling live cells in the same way to calcein, AM. It has a maximum excitation at 405 nm, which perfectly matches the violet laser line equipped in most flow cytometers, and it is well-excited by the excitation sources of fluorescence microscopes. Upon getting into live cells the weakly fluorescent CytoCalcein™ Violet 450 is hydrolyzed into a strongly fluorescent dye that has an excitation/emission maxima of 405/450 nm. This exceptional spectral separation from the typical FACS fluorophores provides additional options for multiplexing experiments. Compared to calcein blue, CytoCalcein™ Violet 450 is brighter and is be better excited by the 405 nm laser line. CytoCalcein™ Violet 450 and CytoCalcein™ Violet 500 have been developed for flow cytometric applications. CytoCalcein™ dyes exhibit similar biological properties to calcein, AM. They are optimized for the excitation wavelengths of a variety of flow cytometers, providing additional colors for flow cytometric analysis of live cells. CytoCalcein™ Violet 450 and CytoCalcein™ Violet 500 are well excited by 405 nm of violet laser and emit fluorescence at 450 nm and 500 nm respectively.

Additional Text

Storage Note

Freeze (< -15 °C); Minimize light exposure;|12 months