MycoLight™ Bacterial Viability Assay Kit

Catalogue Number: 22400-AAT

Manufacturer:	AAT Bioquest
Shelf Life:	6 months
Type:	Cell Proliferation / Viability Assays
Shipping Condition:	RT
Unit(s):	200 Tests

Overview
Datasheets

Description

Description: AAT Bioquest's Mycolight™ Bacterial Viability Assay Kit provides two-color fluorescence assay of bacterial viability in both gram-positive and negative bacterial cell. The kit utilizes the mixture of our green fluorescent nucleic acid stain MycoLight™ Green and the red-fluorescent nucleic acid stain propidium iodide. When used alone, the MycoLight™ Green stain generally labels all bacteria (live and dead) in a population. In contrast, propidium iodide penetrates only bacteria with damaged membranes, causing a reduction in the MycoLight™ Green stain fluorescence when both dyes are present. Thus, with an appropriate mixture of the MycoLight™ Green and propidium iodide stains, live bacteria with intact cell membranes emits green fluorescence, whereas dead or dying bacteria with damaged membranes gives red fluorescence. The Mycolight ™ Bacterial Viability Assay Kit is a robust tool for monitoring the viability of bacterial populations as a function of the membrane integrity of the cell. Stained cells can be monitored fluorimetrically at 510-530 nm (FITC filter) and 600-660 nm (Texas red filter) with excitation at 488 nm, the most common excitation light source.

Additional Text

Storage Note

Freeze (< -15 °C); Minimize light exposure;|6 months

22400 Protocol

22400 MSDS

Price

£335.00

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