MycoLight™ Fluorescence Live/Dead Bacterial Imaging Kit
Catalogue Number: 22411-AAT
| Manufacturer: | AAT Bioquest |
| Type: | Cell Proliferation / Viability Assays |
| Shipping Condition: | RT |
| Unit(s): | 100 tests |
Description
Description: AAT Bioquest's MycoLight™ Fluorescence Live/Dead Bacterial Imaging Kit provides two-color fluorescence assay for visualizing live and dead bacteria through fluorescent microscope. MycoLight™ 520 is a non-fluorescent esterase substrate that diffuses into both Gram positive and Gram-negative bacteria. Upon hydrolysis by bacterial intracellular non-specific esterases, a green fluorescent product is produced and accumulated within bacteria. In contrast, propidium iodide is a red-fluorescent nucleic acid stain that only penetrates bacteria with damaged membranes. Thus, with an appropriate mixture of the MycoLight™ 520 and propidium iodide stains, live bacteria with intact cell membranes emits green fluorescence, whereas dead bacteria with damaged membranes gives red fluorescence. The MycoLight™ Fluorescence Live/Dead Bacterial Imaging Kit is a robust tool for imaging Live/Dead bacteria. Stained cells can be monitored fluorimeterically (FITC filter set) and (TRITC filter set) for live and dead bacteria respectively.
Additional Text
Storage Note
Multiple;|12 months
22411 Protocol
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations